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Counter-regulatory cytokine responses in serum and injured brain tissue following polytrauma. ( A ) Serum IL-4. ( B ) IL-4 in injured brain tissue. ( C ) Serum <t>IL-10.</t> ( D ) IL-10 in injured brain tissue. ( E ) Serum TGF-β. ( F ) TGF-β in injured brain tissue. ELISA: n = 6 per group at each time point. Brain-tissue values were normalized to total protein. Data are mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001; ns—not significant.
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Counter-regulatory cytokine responses in serum and injured brain tissue following polytrauma. ( A ) Serum IL-4. ( B ) IL-4 in injured brain tissue. ( C ) Serum IL-10. ( D ) IL-10 in injured brain tissue. ( E ) Serum TGF-β. ( F ) TGF-β in injured brain tissue. ELISA: n = 6 per group at each time point. Brain-tissue values were normalized to total protein. Data are mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001; ns—not significant.

Journal: Biology

Article Title: Temporal Evolution and Dissociation of Neuroendocrine Stress Inflammatory Responses Neurological Dysfunction and Multiorgan Injury Following Experimental Polytrauma in Mice

doi: 10.3390/biology15191788

Figure Lengend Snippet: Counter-regulatory cytokine responses in serum and injured brain tissue following polytrauma. ( A ) Serum IL-4. ( B ) IL-4 in injured brain tissue. ( C ) Serum IL-10. ( D ) IL-10 in injured brain tissue. ( E ) Serum TGF-β. ( F ) TGF-β in injured brain tissue. ELISA: n = 6 per group at each time point. Brain-tissue values were normalized to total protein. Data are mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001; ns—not significant.

Article Snippet: Concentrations of IL-1β, IL-6, TNF-α, IL-4, IL-10 and TGF-β in peripheral serum and injured brain tissue homogenates were determined using enzyme-linked immunosorbent assay kits (CSB-E08054m, CSB-E04639m, CSB-E04741m, CSB-E04634m-IS, CSB-E04594m-IS, CSB-E04726m-IS, CUSABIO, https://www.cusabio.com/ ) according to the manufacturer’s instructions.

Techniques: Tissue, Enzyme-linked Immunosorbent Assay